015 Clonal sharing of skin T cells within different skin compartments and blood
نویسندگان
چکیده
The human skin harbors a heterogenous pool of cytotoxic tissue-resident memory T (TRM) cells seeded during infections and inflammation. TRM are retained at the site viral provide local immunity. These persist for years in after bone marrow transplantation but circulating ex-TRM indicate several potential fates. Here, we explored clonal relationships between different compartments circulation. 10x Genomics 5 prime immune profiling coupled with feature barcoding technology was employed to investigate relationship, transcriptomic proteomic profiles single sorted from blood, subcutis, dermis, epidermis. A few CD8+ cell-clones dominate epidermis CD8+CD103+ CD49a+ showed least diverse cell receptor (TCR) repertoire. Clonal overlap found all three compartments: blood cells, dermal epidermal cells. highest degree detected dermis. Clones overlapping scarce highly abundant clones. Our results suggest that lowly clonotypes have circulatory counterpart, which can react reinfection secondary sides, whereas clones might be specific.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2022
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2022.09.024